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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in many tumors, which ends up in chromosome instability, and therefore may add to tumorigenesis. This protein was located to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important job in promoting mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants were being documented but specific nucleotide sequence details is not however available.
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Blocking peptides are peptides that bind specifically for the goal antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guide outlines further probable optimization of mobile and immunoassay parameters to be certain the absolute best success are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the very best effects are...
Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, you can see which staining is certain.
Concentrate on Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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